<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1222" dy="693" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：静息E. coli实现醇胺化级联，避免酶纯化与外源NAD+" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：共表达ADH与AmDH，构建胞内氢借位级联" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="54" y="214" width="313" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造：优化基因构建、葡萄糖调控NAD+/NADH" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFE6CC;strokeColor=#D79B00;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="46" y="388" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="结果：82%转化率，&gt;99% ee，克级制备成功" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="64" y="562" width="292" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结论：绿色高效醇胺化新路径，操作简化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D4E6F1;strokeColor=#2E86C1;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="71" y="736" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="node1" target="node2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="node2" target="node3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="node3" target="node4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="node4" target="node5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>