<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="研究问题：JA和激发子如何调控Str基因表达？" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D6EAF8;strokeColor=#2E86C1;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="464" y="40" width="313" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" value="策略：启动子缺失分析+酵母单杂交+EMSA+瞬时共转化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5F5E3;strokeColor=#27AE60;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" value="关键改造①：Str启动子缺失突变体与阻断突变(M2-M5)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDEBD0;strokeColor=#E67E22;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" value="关键改造②：JERE元件(RV区42bp)鉴定及四聚体实验" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDEBD0;strokeColor=#E67E22;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" value="关键改造③：酵母单杂交筛选ORCA1/ORCA2及EMSA验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDEBD0;strokeColor=#E67E22;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" value="结果：ORCA2结合JERE激活Str启动子2.9倍，响应MeJA/激发子" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E8DAEF;strokeColor=#8E44AD;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" value="结论：JERE(含GCC-box样序列)+ORCA2构成JA响应关键调控模块" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F2D7D5;strokeColor=#C0392B;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;shadow=0;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#2E86C1;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#27AE60;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#27AE60;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#27AE60;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E67E22;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E67E22;" edge="1" parent="1" source="node4" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E67E22;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#8E44AD;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>