<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1426" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="研究问题：解析铁皮石斛PLBs中生物碱在前体和MeJA处理下的积累机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略：前体+MeJA处理PLBs（0/4/24h），转录组测序+WGCNA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="关键改造1：总生物碱含量4h达319 μg/g DW（对照189）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键改造2：RNA-Seq获32,727个unigenes；C4/T4和C24/T24共7,698个DEGs" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键改造3：WGCNA鉴定brown模块（885基因）与生物碱含量正相关（r=0.82）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="655" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="关键改造4：关键酶基因显著上调（DoOPR↑1625倍、DoLOX↑29倍等）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n2" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n3" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="结果：6个生物碱合成基因+7个JA合成基因与含量高度相关（|r|&gt;0.8）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n4" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n8" value="结论：13个关键酶基因+13个TF协同调控生物碱积累，提供代谢工程靶标" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="860" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n5" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;" edge="1" parent="1" source="n6" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>