<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="793" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    <mxCell id="node1" value="研究问题：解析JA信号调控青蒿素生物合成机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="186" y="40" width="320" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node2" value="策略：克隆AaMYC2并分析其表达模式" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="221" y="162" width="250" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node3" value="策略：酵母双杂交/BiFC验证蛋白互作" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="218" y="284" width="257" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node4" value="策略：Y1H验证AaMYC2与启动子结合" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="224" y="406" width="243" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node5" value="关键改造：过表达AaMYC2转基因黄花蒿" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="264" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node6" value="关键改造：RNAi沉默AaMYC2转基因黄花蒿" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="374" y="528" width="278" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node7" value="结果：过表达使青蒿素含量增加23%-55%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="271" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node8" value="结果：RNAi后青蒿素含量降低至54%-81%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="381" y="650" width="271" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node9" value="结论：AaMYC2作为正调控因子激活青蒿素合成" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="193" y="772" width="306" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node4" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node6" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;fontColor=#000000;strokeColor=#000000;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>