<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="研究问题：视黄酸合成缺乏生物催化路线" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="266" y="40" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="策略：筛选细菌同源ALDH酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="300" y="162" width="210" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="关键改造：克隆表达BcALDH" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="300" y="284" width="210" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="关键改造：定点突变E194S/E457V" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="290" y="406" width="229" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="结果：NAD⁺氧化视黄醛生成视黄酸（转化率53%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="50" y="528" width="334" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="结果：NADPH还原视黄醛生成视黄醇（突变体效率提升）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="结果：人ALDH1A1 E196S视黄醇产量提升4.6倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="454" y="528" width="306" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="结论：BcALDH为首个细菌视黄醛双向转化酶工具" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="650" width="320" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" source="node1" target="node2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" source="node2" target="node3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" source="node3" target="node4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node4" target="node5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node5" target="node6" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node4" target="node7" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" source="node6" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" source="node7" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>