<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="960" dy="592" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="N0" value="研究问题：氮素对藤仓镰孢霉类胡萝卜素及次级代谢的调控机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=11;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E01" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N0" target="N1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N1" value="实验策略：富氮/低氮培养野生型与carS突变体，氮饥饿时间序列分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=11;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E12" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N1" target="N2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N2" value="关键改造：carS去调控突变体（SG1/SG22/SG36/SG39）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=11;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E23" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N2" target="N3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N3" value="检测方法：HPLC测GA3，分光光度法测比卡维林/类胡萝卜素，RT-PCR分析基因表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=11;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E34" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N3" target="N4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N4" value="关键结果1：高氮抑制类胡萝卜素，低氮诱导合成（低氮48 vs 高氮10 μg/g）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E45" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N4" target="N5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N5" value="关键结果2：carS突变体比卡维林显著升高（SG1高达20倍），GA3产量降低" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E56" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N5" target="N6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N6" value="关键结果3：氮饥饿诱导carRA/carB约10倍，ggs1同步上调，光照协同诱导" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="772" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="E67" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=10;" edge="1" parent="1" source="N6" target="N7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="N7" value="结论：氮负调控类胡萝卜素，CarS可能为转录抑制因子，连接类胡萝卜素与氮调控次级代谢网络" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=11;fontStyle=1;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="894" width="340" height="64" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>