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<mxCell id="node2" value="策略：敲除4个基因+适应性进化构建ECOM4/ECOM4LA菌株&#10;基因：cydAB（细胞色素bd氧化酶）cyoABCD（bo3氧化酶）cbdAB（bd-II氧化酶）ygiN（奎醇单加氧酶）→ 末端氧化酶+Quinone单加氧酶缺失" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
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<mxCell id="node3" value="关键改造：敲除cydAB cyoABCD cbdAB ygiN，醌库由泛醌→甲基萘醌，ArcB/ArcA激活" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
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<mxCell id="node4" value="结果1：氧摄取率降至1/60，有氧/无氧均产D-乳酸（98%/92%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
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<mxCell id="node5" value="结果2：ArcA regulon有氧显著富集，TCA通量≈0，PPP通量仅2%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
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<mxCell id="node6" value="结果3：wrbA↑40倍、frdABCD↑50倍、sdh↓25倍，NADH经甲基萘醌氧化&#10;生理表征：有氧生长率0.32/h，无氧0.27/h；甲基萘醌450±100 nmol/g DCW；13C示踪显示OAA输入99.5%来自厌补反应" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
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