<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="拉格酵母AGT1基因缺陷导致高浓度麦汁发酵缓慢" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFE6CC;strokeColor=#D79B00;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="50" y="40" width="320" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="从艾尔酵母A60克隆功能性AGT1基因并整合至拉格酵母A15基因组" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="修复AGT1基因并受组成型PGK1启动子驱动，同源重组整合至AGT1基因座" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="麦芽三糖-抗霉素A选择性平板筛选转化子并验证AGT1表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="麦芽糖/麦芽三糖转运活性显著提高（1.6→5.1-10.3 U/g；3.4→5.1-8.5 U/g）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="24°P麦汁发酵时间缩短11%-37%，乙醇产量提高至95-96 g/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="残留麦芽糖降低1.5 g/L，麦芽三糖降低4-7 g/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="50" y="772" width="320" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="110-112代非选择性培养后基因型和表型保持稳定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="46" y="894" width="327" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node9" value="修复AGT1并组成型表达可显著改善高浓度酿造性能，且不改变酵母其他特性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="1016" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>