<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="793" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="问题：鉴定特异性转移GalNAc至Und-P的新酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="638" y="40" width="299" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：体内互补+体外酶活+拓扑预测+系统发育分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="617" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造①：AH-3△wecP补回WecP完全恢复O34抗原" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造②：E. coli △wecA共表达WecP+Gne恢复O7抗原（含GalNAc）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="关键改造③：K. pneum △wecA共表达WecP+Gne恢复O1抗原（含GalNAc）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="860" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="关键改造④：体外放射性标记酶活测定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1270" y="388" width="264" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果：WecP特异性催化GalNAc转移，不识别GlcNAc；与WecA同源性&lt;30%，拓扑与WbaP相似" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="617" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：WecP代表UDP-HexNAc:polyprenol-P转移酶家族的新亚组" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="617" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node3" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node4" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>