<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="0" dy="0" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：DOXP途径基因必需性与转录调控机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="255" y="40" width="320" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：基因敲除/互补、过表达、RT-PCR、报告基因等系统验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="245" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造：dxs1/gcpE基因敲除与互补菌株构建（merodiploid系统）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="245" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造：Dxs过表达与启动子活性检测（dxr-gcpE操纵子分析）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d9e2f3;strokeColor=#8eaadb;fontSize=12;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="245" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果：dxs1/gcpE必需，dxr必需并定位功能起始位点" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="245" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果：过表达Dxs1上调dxr-gcpE转录，HMB-PP增加2倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="40" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果：dxr上游启动子活性高，gcpE无独立启动子（共转录）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="57" y="1084" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：DOXP途径为结核菌必需，GcpE限速，Dxs调控通量" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f5f5f5;strokeColor=#666666;fontSize=13;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="450" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="应用：DOXP途径为抗结核药物开发特异性靶点" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;absoluteArcSize=2;verticalAlign=middle;align=center;arcSize=8;" parent="1" vertex="1">
      <mxGeometry x="467" y="1084" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" parent="1" source="node1" target="node2" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" parent="1" source="node2" target="node3" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;dashed=1;" parent="1" source="node3" target="node4" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" parent="1" source="node4" target="node5" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" parent="1" source="node5" target="node6" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" parent="1" source="node6" target="node7" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;dashed=1;" parent="1" source="node5" target="node8" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" parent="1" source="node8" target="node9" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#b85450;dashed=1;" parent="1" source="node6" target="node8" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#b85450;dashed=1;" parent="1" source="node7" target="node8" edge="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>