<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="n1" value="研究问题：HMG-CoA合酶是否参与IV-CoA替代生物合成？" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;absoluteArcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n2" value="策略：构建bkd缺失及mvaS敲除突变体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;absoluteArcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="82" y="214" width="257" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n3" value="关键改造：多突变体组合（Δbkd、Δbkd mvaS::kan、mvaS::kan）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;absoluteArcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n4" value="结果：双突变体iso15:0降至3.8%（野生型41.9%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;absoluteArcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="43" y="562" width="334" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n5" value="结论：MvaS参与IV-CoA替代途径，子实体形成期诱导，受IVA反馈抑制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;absoluteArcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="n1" target="n2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="n2" target="n3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="n3" target="n4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" source="n4" target="n5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>