<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="研究问题：miRNA如何调控小蓟萜类生物合成？" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="248" y="40" width="313" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node2" value="策略：尖孢镰刀菌诱导+多组学分析" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="284" y="162" width="243" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node3" value="SPME GC-MS代谢物谱分析，确定6dpi最优" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="126" y="284" width="278" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node4" value="小RNA文库构建与高通量测序" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="474" y="284" width="210" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node5" value="鉴定58个差异表达miRNA及2个新miRNA" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffffcc;strokeColor=#d6b656;fontSize=12;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="276" y="406" width="257" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node6" value="psRobot靶标预测+GO注释+降解组测序验证" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="58" y="528" width="285" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node7" value="RT-qPCR验证：6个miRNA靶向MVA/MEP途径关键酶" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="40" y="650" width="320" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node8" value="结果：α-雪松烯等萜类显著积累，miRNA-MVD/HMGR/DXS等调控网络" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="412" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node9" value="结论：miRNA可作为调控小蓟萜类生物合成的基因工具" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;">
      <mxGeometry x="430" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge3" edge="1" parent="1" source="node2" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge4" edge="1" parent="1" source="node4" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge5" edge="1" parent="1" source="node3" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge6" edge="1" parent="1" source="node5" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge7" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge8" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge9" edge="1" parent="1" source="node5" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge10" edge="1" parent="1" source="node8" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>