<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：橄榄油品质受基因型与环境影响，育种周期长，急需快速鉴定关键基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1F0FF;strokeColor=#4A90D9;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：对橄榄转录组RNA-seq数据开展meta分析，整合多个独立实验的DEGs&#10;橄榄油品质关键基因鉴定 — meta分析研究路线" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1F0FF;strokeColor=#4A90D9;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="73" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="数据筛选：39个转录组实验中选出4个理想实验进行meta分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D4EDDA;strokeColor=#28A745;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="293" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造：整合DEGs，富集分析+与已知油合成基因对比，提高统计功效与可重复性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF3CD;strokeColor=#FFC107;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="415" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="通路富集：半乳糖代谢、糖酵解、丙酮酸代谢、脂肪酸合成等关键通路" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8D7DA;strokeColor=#DC3545;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="537" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="关键基因鉴定：C1/C2/C3中分别鉴定1472/5175/1034个DEGs，含869个新基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8D7DA;strokeColor=#DC3545;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="659" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="关键酶：SAD、FAD2、ACCase、DGAT、PDAT、FabH/F等油合成酶系逐一确认" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8D7DA;strokeColor=#DC3545;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="781" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="功能验证：磷酸葡萄糖变位酶突变体油含量降低约40%，油酸达83%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E2F0D9;strokeColor=#28A745;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="903" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="结论：获得基因特异性标记，缩短橄榄育种筛选周期，提供基因组编辑靶点" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D6EAF8;strokeColor=#2980B9;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="659" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90D9;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90D9;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90D9;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90D9;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#DC3545;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#DC3545;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#DC3545;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#2980B9;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#2980B9;" edge="1" parent="1" source="node5" target="node9">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>